Production of Functional Glucagon-Secreting Alpha Cells from Human Embryonic Stem Cells
نویسندگان
چکیده
Alireza Rezania, Michael J. Riedel, Rhonda D. Wideman, Francis Karanu, Ziliang Ao, Garth L. Warnock, and Timothy J. Kieffer BetaLogics Venture, Centocor Research & Development, Skillman, NJ, USA; Laboratory of Molecular and Cellular Medicine, Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3; Department of Surgery, University of British Columbia, Vancouver, BC, Canada, V5Z 1M9
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Introduction: Type I diabetes mellitus is caused by autoimmune destruction of the insulin-producing β-cells. A new potential method for curing the disease is transplantation of differentiated insulin- secreting cells from human embryonic stem cells. Methods: Human embryonic stem cell lines (Royan H1) were used to produce embryoid bodies. Differentiation carried out by growth factor-mediated se...
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OBJECTIVE Differentiation of human embryonic stem (hES) cells to fully developed cell types holds great therapeutic promise. Despite significant progress, the conversion of hES cells to stable, fully differentiated endocrine cells that exhibit physiologically regulated hormone secretion has not yet been achieved. Here we describe an efficient differentiation protocol for the in vitro conversion...
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